| Singles competition |
| 64 | Lauren O'Malley (John Carroll) def. Kate Lee (Washington University) 6-1, 6-2 |
| 64 | Devin Gramley (Denison) def. Gaby DeLeon (Allegheny College) 7-6 (7-5), 7-5 |
| 64 | Handa, Claire (University of Chicago) def. Natalie Rahrig (Hope College) 6-0, 6-1 |
| 64 | Rainie Heck (Oberlin College) def. Lynn Kelly (Ohio Northern women) 6-0, 6-4 |
| 64 | Julie Lord (Lake Forest College) def. Maria Franco (Kalamazoo College) 6-3, 6-0 |
| 64 | Rudin, Alyssa (University of Chicago) def. Ceylan Can (Kenyon College) 6-3, 6-3 |
| 64 | Claire Hallock (Hope College) def. Lisa Chionis (Washington University) 6-1, 6-1 |
| 64 | Nicole Cardwell (Case Western Reserve) def. Ali Bush (DePauw University) 2-6, 6-3, 6-1 |
| 64 | Diana Aboubakare (Kenyon College) def. Madeline Hart (Denison) 6-1, 6-1 |
| 64 | Nina Cepeda (Case Western Reserve) def. Travis, Adrienne (University of Chicago) 6-1, 6-3 |
| 64 | Grace Deering (Washington University) def. Christina Lombardi (Saint Mary's College), by default |
| 64 | Behrle, Monica (Webster University) def. Jayde Robinson (Baldwin Wallace) 6-0, 6-0 |
| 64 | Libby Jubas (Washington University) def. Sarah Hughes (Oberlin College) 6-4, 1-6, 6-4 |
| 64 | Xu, Catherine (University of Chicago) def. Taylor LaBarge (Hope College) 6-1, 6-2 |
| 64 | Madeleine Paolucci (Case Western Reserve) def. Grace Winslow (Kenyon College) 7-5, 6-0 |
| 64 | Timm, Annie (Augustana) def. Amanda Showalter (DePauw University) 7-5, 3-6, 6-4 |
| 64 | Gutierrez, Laura (University of Chicago) def. ILANA Blackwood (Kenyon College) 6-2, 6-3 |
| 64 | Ashley Lessen (Washington University) def. Schaffer, Caitlyn (Augustana) 1-6, 6-4, 7-6 (7-4) |
| 64 | Peyton Young (Case Western Reserve) def. Claudia Olaes (Oberlin College) 3-6, 6-1, 6-2 |
| 64 | Amanda Bandrowski (Hope College) def. Laura Hanselman (Kalamazoo College) 5-7, 6-1, 6-2 |
| 64 | Ally Persky (Washington University) def. Ellison, Daryn (University of Chicago) 6-1, 6-2 |
| 64 | Halicka, Weronika (Illinois Tech) def. Madisyn Rini (John Carroll) 6-1, 6-1 |
| 64 | Mira Amin (Case Western Reserve) def. Joy Li (College of Wooster) 6-3, 6-0 |
| 64 | Lauren Hawley (Denison) def. Sydney Bergman (Otterbein University) 6-0, 6-4 |
| 64 | Laurel Wanger (Washington University) def. Quincey Glupker (Hope College) 7-5, 6-4 |
| 64 | Alyssa Seneviratne (DePauw University) def. Mayada Audeh (Oberlin College) 1-6, 6-1, 6-1 |
| 64 | Annie Reiner (Kenyon College) def. Leah Newhof (Calvin College) 4-6, 6-1, 6-3 |
| 64 | Antohi, Marjorie (University of Chicago) def. Amanda Ress Liere (Allegheny College) 6-1 |
| 64 | Navarro, Estefania (University of Chicago) def. Megan Hickey (Denison) 6-4, 6-1 |
| 64 | Erika Oku (Kenyon College) def. Sanjna Tripathy (Washington University) 6-0, 2-0, retired |
| 64 | Vilde Olvin (North Park University) def. Angell, Molly (Millikin) 6-1, 6-1 |
| 64 | Nithya Kanagasegar (Case Western Reserve) def. Ashley Ochsenhirt (Ohio Northern women) 6-0, 6-2 |
| 32 | Lauren O'Malley (John Carroll) def. Devin Gramley (Denison) 6-0, 6-1 |
| 32 | Handa, Claire (University of Chicago) def. Rainie Heck (Oberlin College) 6-0, 6-3 |
| 32 | Rudin, Alyssa (University of Chicago) def. Julie Lord (Lake Forest College) 6-4, 6-1 |
| 32 | Claire Hallock (Hope College) def. Nicole Cardwell (Case Western Reserve) 6-4, 6-3 |
| 32 | Diana Aboubakare (Kenyon College) def. Nina Cepeda (Case Western Reserve) 6-2, 6-2 |
| 32 | Grace Deering (Washington University) def. Behrle, Monica (Webster University) 6-2, 6-2 |
| 32 | Libby Jubas (Washington University) def. Xu, Catherine (University of Chicago) 6-3, 6-4 |
| 32 | Madeleine Paolucci (Case Western Reserve) def. Timm, Annie (Augustana) 6-1, 6-0 |
| 32 | Ashley Lessen (Washington University) def. Gutierrez, Laura (University of Chicago) 6-3, 1-6, 6-2 |
| 32 | Amanda Bandrowski (Hope College) def. Peyton Young (Case Western Reserve) 6-2, 6-1 |
| 32 | Halicka, Weronika (Illinois Tech) def. Ally Persky (Washington University) 5-7, 6-4, 6-2 |
| 32 | Lauren Hawley (Denison) def. Mira Amin (Case Western Reserve) 6-4, 7-5 |
| 32 | Laurel Wanger (Washington University) def. Alyssa Seneviratne (DePauw University) 7-5, 7-6 (12-10) |
| 32 | Antohi, Marjorie (University of Chicago) def. Annie Reiner (Kenyon College) 6-0, 6-0 |
| 32 | Erika Oku (Kenyon College) def. Navarro, Estefania (University of Chicago) 6-2, 7-5 |
| 32 | Nithya Kanagasegar (Case Western Reserve) def. Vilde Olvin (North Park University) 6-0, 6-0 |
| 16 | Handa, Claire (University of Chicago) def. Lauren O'Malley (John Carroll) 3-6, 6-4, 6-3 |
| 16 | Claire Hallock (Hope College) def. Rudin, Alyssa (University of Chicago) 6-4, 6-4 |
| 16 | Grace Deering (Washington University) def. Diana Aboubakare (Kenyon College) 6-4, 3-6, 7-5 |
| 16 | Madeleine Paolucci (Case Western Reserve) def. Libby Jubas (Washington University) 0-6, 6-3, 6-4 |
| 16 | Ashley Lessen (Washington University) def. Amanda Bandrowski (Hope College) 6-3, 2-6, 6-3 |
| 16 | Halicka, Weronika (Illinois Tech) def. Lauren Hawley (Denison) 6-2, 6-1 |
| 16 | Antohi, Marjorie (University of Chicago) def. Laurel Wanger (Washington University) 6-0, 6-2 |
| 16 | Nithya Kanagasegar (Case Western Reserve) def. Erika Oku (Kenyon College) 2-6, 7-6 (7-5), 6-2 |
| 8 | Handa, Claire (University of Chicago) def. Claire Hallock (Hope College), by default |
| 8 | Grace Deering (Washington University) def. Madeleine Paolucci (Case Western Reserve) 6-0, 6-1 |
| 8 | Ashley Lessen (Washington University) def. Halicka, Weronika (Illinois Tech) 6-4, 7-5 |
| 8 | Antohi, Marjorie (University of Chicago) def. Nithya Kanagasegar (Case Western Reserve) 6-0, 6-0 |
| SF | Handa, Claire (University of Chicago) def. Grace Deering (Washington University) 6-3, 6-3 |
| SF | Antohi, Marjorie (University of Chicago) def. Ashley Lessen (Washington University) 6-2, 7-6 (9-7) |
| F | Handa, Claire (University of Chicago) vs. Antohi, Marjorie (University of Chicago) -- no result |